Journal: bioRxiv

Article Title: Structural, functional and computational studies of membrane recognition by Plasmodium Perforin-Like Proteins 1 and 2

doi: 10.1101/2021.12.16.473088

Figure Lengend Snippet: (A) Representative lipid dot-blots. APCβs were incubated with commercially available nitrocellulose membrane dotted with common mammalian membrane lipid species. (B-C) Liposome sedimentation assays of APCβ domains of PvPLP1 (B) and PvPLP2 (C). All liposomes are composed of 40% phosphatidylcholine (PC), 30% phosphatidylethanolamine (PE) and 30% of varied lipids. CHOL, Cholesterol; Brain Ex , Brain Total lipid Extract; Liver Ex , Liver Total Lipid Extract; SM, Sphingomyelin; CL, cardiolipin; PS, phosphatidylserine; Egg-PA, L-α-phosphatidic acid; Lyso-PS, L-α-lysophosphatidylserine.

Article Snippet: POPC (1-palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine), POPE (1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoethanolamine), Cholesterol, Brain Total lipid Extract, Liver Total Lipid Extract, Sphingomyelin (brain, Porcine), Cardiolipin (bovine heart), POPS (1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoserine), Egg-PA (L-α-phosphatidic acid, LPA) and Lyso-PS (L-α-lysophosphatidylserine) were purchased from Avanti Polar Lipids Inc. Liposomes with indicated compositions were prepared as described [ ].

Techniques: Incubation, Sedimentation

Journal: Langmuir : the ACS journal of surfaces and colloids

Article Title: Determining Molecular-Level Interactions of Carboxyl-Functionalized Nanodiamonds with Bacterial Membrane Models as the Basis for Antimicrobial Activity.

doi: 10.1021/acs.langmuir.4c05173

Figure Lengend Snippet: Figure 1. Schematic illustration for (A) E. coli (Gram-negative) and (B) S. aureus (Gram-positive) bacteria along with their respective membrane structures (insets). The lipid membrane regions mimicked in this study are indicated, and the molecular structures of the lipids in the composition of (A) E. coli lipid extract and (B) S. aureus lipid mixture are shown. The numbers 1 and 2 refer to the lipid chains of PE and PG, which can have different sizes and be unsaturated or not.

Article Snippet: Escherichia coli (E. coli) total lipid extract (strain B; ATCC 11303; Avanti Polar Lipids, 100500P) consists of 57.5% phosphatidylethanolamines (PE), 15.1% phosphatidylglycerols (PG), 9.8% cardiolipins (CL), and 17.6% unidentified components.

Techniques: Bacteria, Membrane

Journal: Langmuir : the ACS journal of surfaces and colloids

Article Title: Determining Molecular-Level Interactions of Carboxyl-Functionalized Nanodiamonds with Bacterial Membrane Models as the Basis for Antimicrobial Activity.

doi: 10.1021/acs.langmuir.4c05173

Figure Lengend Snippet: Figure 2. Surface pressure (mN/m) versus area (102 cm2/mL lipid extract) isotherms and surface compression modulus (Cs −1) for (A) E. coli and (B) E. coli:NDCOOHs (1:1, 1:2, and 1:4, v/v) and for (C) S. aureus and (D) S. aureus:NDCOOHs monolayers (1:1, 1:2, and 1:4, v/v).

Article Snippet: Escherichia coli (E. coli) total lipid extract (strain B; ATCC 11303; Avanti Polar Lipids, 100500P) consists of 57.5% phosphatidylethanolamines (PE), 15.1% phosphatidylglycerols (PG), 9.8% cardiolipins (CL), and 17.6% unidentified components.

Techniques:

Journal: Langmuir : the ACS journal of surfaces and colloids

Article Title: Determining Molecular-Level Interactions of Carboxyl-Functionalized Nanodiamonds with Bacterial Membrane Models as the Basis for Antimicrobial Activity.

doi: 10.1021/acs.langmuir.4c05173

Figure Lengend Snippet: Figure 3. PM-IRRAS spectra for (A) E. coli and E. coli:NDCOOHs (1:1) and (B) S. aureus and S. aureus:NDCOOHs (1:2) monolayers on the PBS subphase at 30 mN/m. The left panels highlight bands related to polar head groups, while the right panels refer to those associated with alkyl chain groups.

Article Snippet: Escherichia coli (E. coli) total lipid extract (strain B; ATCC 11303; Avanti Polar Lipids, 100500P) consists of 57.5% phosphatidylethanolamines (PE), 15.1% phosphatidylglycerols (PG), 9.8% cardiolipins (CL), and 17.6% unidentified components.

Techniques:

Journal: Langmuir : the ACS journal of surfaces and colloids

Article Title: Determining Molecular-Level Interactions of Carboxyl-Functionalized Nanodiamonds with Bacterial Membrane Models as the Basis for Antimicrobial Activity.

doi: 10.1021/acs.langmuir.4c05173

Figure Lengend Snippet: Figure 4. Schematic representation of the binding mechanism of NDCOOHs in (A) E. coli extract and (B) S. aureus lipid mixture monolayers. Green arrows indicate attractive electrostatic interactions, red arrows show repulsive electrostatic interactions, and blue dashed arrows illustrate secondary interactions, such as van der Waals and hydrogen bonding.

Article Snippet: Escherichia coli (E. coli) total lipid extract (strain B; ATCC 11303; Avanti Polar Lipids, 100500P) consists of 57.5% phosphatidylethanolamines (PE), 15.1% phosphatidylglycerols (PG), 9.8% cardiolipins (CL), and 17.6% unidentified components.

Techniques: Binding Assay

Journal: Langmuir : the ACS journal of surfaces and colloids

Article Title: Determining Molecular-Level Interactions of Carboxyl-Functionalized Nanodiamonds with Bacterial Membrane Models as the Basis for Antimicrobial Activity.

doi: 10.1021/acs.langmuir.4c05173

Figure Lengend Snippet: Figure 5. Comparative evolution of relative area (A/A0) versus time (100 min) for (A) E. coli and E. coli:NDCOOHs (1:1) and for (B) S. aureus and S. aureus:NDCOOHs (1:2) recorded at constant pressure of 30 mN/m. A0 is the extrapolated area of the isotherms at 30 mN/m down to zero pressure.

Article Snippet: Escherichia coli (E. coli) total lipid extract (strain B; ATCC 11303; Avanti Polar Lipids, 100500P) consists of 57.5% phosphatidylethanolamines (PE), 15.1% phosphatidylglycerols (PG), 9.8% cardiolipins (CL), and 17.6% unidentified components.

Techniques:

Journal: Journal of colloid and interface science

Article Title: The influence of steroidal and triterpenoid saponins on monolayer models of the outer leaflets of human erythrocytes, E. coli and S. cerevisiae cell membranes.

doi: 10.1016/j.jcis.2019.12.014

Figure Lengend Snippet: Fig. 1. E. coli (a) and S. cerevisiae (b) phospholipid profiles of the total lipid extracts used in the experiments. Charts constructed using the composition provided by the producer (Avanti Polar Lipids).

Article Snippet: E6510, purity 75%; – Cholesterol, Sigma Aldrich, cat. no. 26732, purity 99%; – SM, Avanti Polar Lipids, cat. no. 860061P, purity > 99%; – TopFluor PC, Avanti Polar Lipids, cat. no. 810281C, purity > 99%; – E. coli Extract Total, Avanti Polar Lipids, cat. no. 100500P, no information about purity; – Yeast Extract Total, Avanti Polar Lipids, cat. no. 190000, no information about purity.

Techniques: Construct